Composite

Part:BBa_K4817009:Design

Designed by: Yexi Liang   Group: iGEM23_SCU-China   (2023-10-11)


IPTG-induced kill switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1813
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1263


Design Notes

The IPTG-induced suicide switch is characterized by the fact that the ccdB ( BBa_P1010), which encodes the toxin, is placed under LacO/LacI control.


Source

<E.coli>

References